|
KMID : 1134820110400040525
|
|
Journal of the Korean Society of Food Science and Nutrition
2011 Volume.40 No. 4 p.525 ~ p.530
|
|
|
Effect of Pine (Pinus densiflora) Needle Extracts on Antioxidant Activity and Proliferation of Osteoclastic RAW 264.7 Cells
|
|
Jeon Min-Hee
Park Mi-Ra
Park Yong-Soo
Hwang Hyun-Jung
Kim Sung-Gu
Lee Sang-Hyeon
Kim Mi-Hyang
|
|
|
Abstract
|
|
|
|
Pine needles have long been used as a traditional health-promoting medicinal food in Korea. This study was carried out to investigate the effects of pine needle extracts on the antioxidant activity, and proliferation of osteoclastic RAW 264.7 cells. Pine needle extracts were examined using hot water, ethanol, hexane, hot water-ethanol, and hot water-hexane. The effects of the pine needle extracts were examined by comparing the results with that of a commercial agents, proanthocyanidin. Analysis of each extract indicated that hot water-ethanol and ethanol extracts contained the highest total polyphenol concentrations. The hot water-ethanol and ethanol extracts also showed relatively the highest SOD-like activity. The proliferation of osteoclastic RAW 264.7 cells treated with pine needle extracts was decreased by lower than 70%. In addition, the hot water and ethanol extracts of pine needle significantly reduced the number of tartrate-resistant acid phosphatase-positive (TRAP+) multinucleated cells from osteoclatic RAW 264.7 cells. These results indicate that pine needle extracts had an anabolic effect on bone through the promotion of osteoclast differentiation, suggesting that they could be used for the treatment of common metabolic bone diseases.
|
|
|
KEYWORD
|
|
|
pine(Pinus densiflora) needle, antioxidant activity, RAW 264.7 cell, MTT assay, TRAP
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|